|
KMID : 0350519920450031079
|
|
Journal of Catholic Medical College
1992 Volume.45 No. 3 p.1079 ~ p.1087
|
|
|
Comparision of Percutaneous Absorption of Methyl Nicotinate with laser Doppler Velocimeter after Sodium Lauryl Sulfate, Cellopohane Tape-Stripping Delipidization Induced Skin Irritations
|
|
|
|
|
Abstract
|
|
|
It is known that stratum corneum is the main barrier to the percutaneous absorption of various chemicals and that percutaneous absorption increases after the deterioration of barrier function by irritant substance. In order to investigate the
role
of
stratum corneum and its lipid component in percutaneous absorption, we experimentally induced irritation on the volar surface of the forearm of 10 healthy volunteers. For this purpose, We used three basic methods; patch-testing with 5% sodium
lauryl
sulfate(SLS) for 24 hours, stripping the skin with cellophane tape and delipidizing stratum corneum with an acetone/ether mixture(1:1 in v/v).
This study was designed to characterize the difference of pharmacokinetics in percutaneous absorption of methyl nicotinate(MN) between normal and damaged skin. After topical application of 10mM MN for 1.5 minutes on the irritated skin sites, we
measured
the cutaneous blood flow employing laser Doppler velocimeter(LDV) in the following time sequence:0(immediately after topical MN application), 1, 3, 5, 10, 15, 20, 30, 40, 50, 60minutes. We then determined the percutaneous absorption pattern of MN
through these measurements.
@ES The results were as follows:
1. The 5% SLS, cellophane tape-stripping and delipidization of stratum corneum induced skin irritation and the cutaneous blood flow monitored with LDV increased, as compared with the normal control group(P<0.05).
2. In 5% SLS-irritated skin group, the percutaneous absorption of MN, as compared with the normal control group, was significantly increased up to 5 minutes(P<0.05). After 5 minutes, however, there was no statistical difference in percutaneous
absorption between the SLS-irritated and the normal control group.
3. In the cellophane tape-stripped skin group, the percutaneous absorption of MN was significantly increased to only 1 minute. After 30 minutes, it decreased significantly more than the normal control group(P<0.05).
4. In the delipidized skin group with the acetone/ether, as compared with the normal control group, the percutaneous absorption of MN was significantly decreased between 5 minutes and 15 minutes(P<0.05), but there was no significant statistical
difference in percutaneous absorption of MN at any other time.
5. Among cellophane tape-stripped skin group, delipidized skin group and normal control group, the cellophane tape-stripped skin group reached the peak response time of blood flow earlier than the other two groups, therefore MN was absorbed most
rapidly in the cellophane tape-stripped skin group.
These results suggest that, in percutaneous absorption, the stratum corneum is the most important rate limiting step and its removal or irritation result in increased and rapid absorption of MN.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|
|